<i>SET domain containing protein 8 (SET8)</i> promotes tumour progression and indicates poor prognosis in patients with laryngeal squamous cell carcinoma

Abstract Objectives SET Domain Containing Protein 8 (SET8), a member of the domain containing methyltransferase family involved in several biological processes and SET8 expression levels, reportedly affects outcomes patients with breast cancer, renal prostate carcinoma, oesophageal squamous cell carcinoma. However, there have been no relevant studies on biofunction use prediction laryngeal carcinoma (LSCC) outcomes. Methods In our study, levels were detected using immunohistochemical staining, western blotting, quantitative real-time RT-PCR (qRT-PCR) semi-quantitative analysis for cancer Additionally, we assessed influence behaviour cells vitro , counting kit-8, clone formation, wound healing, Transwell invasion assays. We subsequently performed qRT-PCR blotting an in-depth study SET8. Results Our showed marked upregulation tumour tissues lines. High predicts poor prognosis LSCC, its can be used as independent predictor LSCC outcome. Subsequent functional analyses indicated that knockdown exerted inhibitory effect proliferation, migration, invasiveness vitro. Conclusions may associated epithelial-mesenchymal transition. results demonstrate higher is unfavourable prognostic exerts tumour-promoting effects LSCC.